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KMID : 0903519940370040248
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1994 Volume.37 No. 4 p.248 ~ p.254
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Molecular Breeding of Herbicide Resistant Transgenic Plants with Bromoxynil Specific Nitrilase gene
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Abstract
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Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic, plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.
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